a. The statistics results shown here are calculated based on clean data after low-quality filter and barcode-split filter.
b. The mapping rate and paired mapping rate are the proportion of read which is mapped or paired-mapped to reference genome.
c. The mismatch means the unmapped bases between read and reference genome.
d. The duplication means duplicated reads introduced by PCR or other processes.
e. The average sequencing depth is calculated by mapped reads without duplicated reads.
f. The coverage is calculated as proportion of reference genome covered by more than 1, 4, 10 or 20 folds reads.
g. The mean insert size is the mean insert size of paired mapped reads.
Insert size distribution (x-axis is insert size, y-axis is the count of paired reads at a certain insert size).
Cumulatived sequencing depth distribution (x-axis is the depth, y-axis is the proportion of reference genome that achieves at or above certain depth).
Sequencing depth distribution (x-axis is the depth, y-axis is the proportion of reference genome at a certain depth).